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Angiotensin Protocols by Donna H. Wang

By Donna H. Wang

Michigan kingdom Univ., East Lansing. interprets wisdom into reproducible equipment than allow biomedical researchers to discover the position of angiotensin in healthiness and sickness. Covers genetic manipulation of specific genes. contains tools for practical reviews of the renin-angiotensin method. DNLM: Renin - Angiotensin System-physiology

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And Soriano, P. (1997) Disruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta-galactosidase in mouse embryos and hematopoietic cells. Proc. Natl. Acad. Sci. USA 94, 3789–3794. 17. Villarreal, F. , Kim, N. , Ungab, G. , Printz, M. , and Dillmann, W. H. (1993) Identification of functional angiotensin II receptors on rat cardiac fibloblasts. Circulation 88, 2849–2861. 18. , and Ichikawa, I. (1999) Communication between myocytes and fibroblasts in cardiac remodeling in angiotensin chimeric mice.

When two loxP sites are present in circular DNA, cre-mediated recombination results in the formation of two separate circles, each containing a single loxP site (Fig. 1). One circle contains the DNA outside the loxP sites, whereas the second circle contains the DNA lying between the loxP sites. When two loxP sites are present in genomic DNA, cre-mediated recombination results in a deletion of DNA lying between two loxP sites and the formation of a circular intermediate containing one loxP site and the intervening sequence (Fig.

Hungary) 9. Micropipets: We made them by pulling out siliconized 100-µL glass micropipets (MICROPET, Clay Adams, Cat. No. 4625) in a gas burner flame. We made three sizes of pipets: thin (inner diameter (id) less than 125 µm), medium (125–150 µm), and thick (around 150 µm). Thin pipets were used for transferring embryos into acidic Tyrode’s solution, medium ones were used for general purposes, and the thick ones were flame polished at the end and used for transferring embryos into the uterus. These pipets were sterilized by heating at 200°C for 2 h.

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