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Advances in Low-Temperature Biology, Volume 3 by Peter L. Steponkus

By Peter L. Steponkus

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_ Jraud Pre Post 1 21 CK-MM (Muscle) D __. m COO 60 E ^ E —- CK-BB (Brain) 2 3 7 14 Sample (days) 21 CK-MB (Heart) 80 70 60 50 40 1200 1000 800 600 30 20 400 200 10 Pre Post 1 2 3 7 Sample (days) 14 21 l^^wJffTiHKaWa^^taapr Pre Post 1 2 3 7 14 Sample (days) 21 Figure 12, Serum enzyme profiles for creatine kinase ancJ its isozymes for muscle, heart and brain in (dogs following ultraprofoun(d hypothermia and blood substitution with Hypothermosol. Mean changes are shown for comparison of group I dogs (perfused with HTS-P and HTS-M) with group II dogs perfused with HTS-P alone.

Following cardiac arrest, the cold solutions were continuously circulated for three hours by the extracorporeal pump, flow rate = 40 to 85 ml/kg/min, MABP: 25 to 40 mm Hg. Intraoperative monitoring included systemic arterial, central venous, ICP, CPP and pulmonary artery wedge blood pressures; heart rate; ECG; urinary output; brain, esophageal and subcutaneous temperatures; and pump flow rates. The blood or perfusate was frequently sampled for blood gases and electrolyte analysis. , 1993). j. M.

Again, in sharp contrast, the mean elevations of these enzymes in surviving dogs from Group II were significantly higher and in many cases proportional changes were an order of magnitude higher than comparable samples from Group I dogs. Moreover, the raised levels of serum CK and its isozymes persisted for several weeks post-operatively in the Group II control dogs. ^ _ Jraud Pre Post 1 21 CK-MM (Muscle) D __. m COO 60 E ^ E —- CK-BB (Brain) 2 3 7 14 Sample (days) 21 CK-MB (Heart) 80 70 60 50 40 1200 1000 800 600 30 20 400 200 10 Pre Post 1 2 3 7 Sample (days) 14 21 l^^wJffTiHKaWa^^taapr Pre Post 1 2 3 7 14 Sample (days) 21 Figure 12, Serum enzyme profiles for creatine kinase ancJ its isozymes for muscle, heart and brain in (dogs following ultraprofoun(d hypothermia and blood substitution with Hypothermosol.

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