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Advances in Applied Microbiology, Vol. 52 by Allen I. Laskin

By Allen I. Laskin

An eclectic quantity of topical reports on all facets of utilized microbiology. It comprises 14 accomplished reports of present learn in utilized microbiology.

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The reporter must be specific for the response of the organism to the molecule that is to be detected and must not be influenced by the presence of potentially competing substances.  A large dynamic range. The ability to detect reporter output over several orders of magnitude is a desirable characteristic to allow for a wide concentration of the target pollutant or environmental condition. 40 HINDE et al.  Appropriateness. Although there are characteristics desirable to most possible applications of reporter genes, specific consideration must be given to the specific application or role of the reporter gene.

Acad. Sci. USA 99, 1581–1586. , and Chen, Z. (2002). Directed evolution of enzmes and pathways for industrial biocatalysis. Curr. Opin. Biotechnol. 13, 104–110. , Bruns, M. , and Tiedje, J. M. (1996). DNA recovery from soils of diverse composition. Appl. Environ. Microbiol. 62, 316–322. This Page Intentionally Left Blank The Potential of Site-Specific Recombinases as Novel Reporters in Whole-Cell Biosensors of Pollution PAUL HINDE, JANE MEADOWS, JON SAUNDERS, AND CLIVE EDWARDS School of Biological Sciences University of Liverpool Liverpool, L69 7ZB United Kingdom I.

Two approaches have been taken to discover esterolytic enzymes from eDNA via high-throughput expression screening. In the first approach, a generic esterase substrate was used to screen a large number of eDNA libraries (Robertson, 1999; Short, 1999a,b, 2001b,c). This substrate is a short-chain fatty acid coupled to a chromophore that allows hydrolytic enzymes to be identified with high sensitivity. In this manner, over 350 unique and novel esterase enzymes were discovered. Bioinformatic analysis of this pool of esterase genes revealed that many of the encoded enzymes clustered into the known families previously reported (Reetz, 2002).

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